Analysis of Food Dyes in Beverages Essay

Purpose To determine the concentration of soil in a sports drink using spectroscopy and interpretical analysis. We result be using beers law to find the undiagnosed concentration of dye in a sports drink. Materials Beakers, 50-mL, 2-3 Test resistances (13 x 100)mm 3-8 FD&C Blue 1 stock answer, 50-mL Lens tissues Pipets, serological, 10-mL Sample of consumer sports drink, 10-mL Spectrophotometer Test pipe rack aim1) Preeminently, follow all laboratory safety guidelines ensuring that you have safety goggle on at all times throughout the lab. 2) gorge the cuvette 2/3 of the demeanor with water.3) Select the appropriate wavelength on the spectrophotometer.4) Place the cuvette in the spectrophotometer and click the calibrate button.5) entrust 5mL of stock ascendant into test tube.6) Fill the cuvette 2/3 of the way with the first solution.7) Place the cuvette in the spectrophotometer.8) deferral a a few(prenominal) split seconds and record the assiduity on your dat a table.9) move 4mL of stock solution and 1mL of water in a test tube to make the second solution.10) Fill the cuvette 2/3 of the way with the second solution.11) Place the cuvette in the spectrophotometer.12) storage area a few seconds and record the submerging on your data table. 13) Put 3mL of stock solution and 2mL of water in a test tube to make the third solution. 14) Fill the cuvette 2/3 of the way with the second solution 15) Place the cuvette in the spectrophotometer.16) Wait a few seconds and record the absorption on your data table. 17) Put 2mL of stock solution and 3mL of water in a test tube to make the fourth solution 18) Fill the cuvette 2/3 of the way with the fourth solution 19) Place the cuvette in the spectrophotometer.20) Wait a few seconds and record the absorption on your data table. 21) Put 1mL of stock solution and 4mL of water in a test tube to make the one-fifth solution 22) Fill the cuvette 2/3 of the way with the fifth solution23) Place the cuvette in the spectrophotometer.24) Wait a few seconds and record the absorption on your data table. 25) After all data are collected, use them to formulate a graph identifying the linear relationship between absorbency and concentration. data and CalculationsData recorded submergence(M)5.634.3443.2882.1721.0860Absorption.462.398.294.186.0820Water (mL)012345Stock issue (mL)543210 linear Regression y = .088x .003A = abca = Molar absorption factorb = Path length of the sample cellc = ConcentrationAbsorbency of Gatorade = 0.1150.115 = (130.000)(1)(c)c = 8.85 x 10-7Conclusion In this experiment, we prepared solutions and used Beers law to analyze the concentration of dye in a beverage. The graph would provide the most accurate means to determine the concentration of an unknown solution and we know that absorbance measurements are most accurate in the run for of A values from 0.2 to 1.0.